Evaluation of Spectrophotometric Methods for Assessing Antioxidant Potential in Plant Food Samples—A Critical Approach

Spectrophotometric antioxidant assays can generally be divided into two fundamental categories: single electron transfer (SET)-based assays and hydrogen atom transfer (HAT)-based methods. In SET-based assays, the progression of the electron exchange reaction is determined by the redox potential of the substrates. In contrast, HAT-based methods assess the antioxidant’s ability to transfer a hydrogen atom to a radical, thereby stabilizing it. The objective of this article is to provide a critical evaluation of antioxidant spectrophotometric assays. Assessing the antioxidant potential of food should involve multiple assays to ensure accuracy and reliability. A positive correlation among different methods enhances…