N4BP3 Activates TLR4-NF-κB Pathway in Inflammatory Bowel Disease by Promoting K48-Linked IκBα Ubiquitination
Central South University · Changsha Medical University · +2 more institutions
Abstract
Lipopolysaccharides (LPS) were used to activate the TLR4 pathway in THP-1/Caco-2 cells. THP-1/Caco-2 cells were transfected with either N4BP3 overexpression or knockdown plasmids, generating N4BP3-overexpressing or N4BP3-deficient cell lines. For in vivo studies, colitis was induced in mice using dextran sodium sulfate (DSS). Additionally, negative control and N4BP3-knockdown C57BL/6 mouse models were established via intraperitoneal injection of control or N4BP3-targeting adeno-associated virus (AAV).
LPS stimulation significantly upregulated N4BP3 expression in THP-1/Caco-2 cells compared to sterile water treatment (P
Citation impact
- FWCI
- 31.89
- Percentile
- 100%
- References
- 45
Authors
13- WJWang JiangCorresponding
Central South University, Changsha Medical University, University of South China
- JYJie Yin
Central South University, Changsha Medical University, University of South China
- MHMinho Han
Central South University, Changsha Medical University, University of South China
- WHWenhua He
First Affiliated Hospital of Jiangxi Medical College
- YZYan Zha
Central South University, Changsha Medical University, University of South China
Topics & keywords
- Ubiquitin
- NF-κB
- Inflammatory bowel disease
- NFKB1
- Signal transduction
- TLR4
- Inflammation
- Immunology