Enrichment of extracellular vesicles using Mag-Net for the analysis of the plasma proteome

Extracellular vesicles (EVs) in plasma are composed of exosomes, microvesicles, and apoptotic bodies. We report a plasma EV enrichment strategy using magnetic beads called Mag-Net. Proteomic interrogation of this plasma EV fraction enables the detection of proteins that are beyond the dynamic range of liquid chromatography-mass spectrometry of unfractionated plasma. Mag-Net is robust, reproducible, inexpensive, and requires 37,000 peptides from >4,000 proteins. Using Mag-Net on a pilot cohort of patients with neurodegenerative disease and healthy controls, we find 204 proteins that differentiate (q-value

• NI
  National Institutes of Health

  Awards: R01 NS115114, F31 AG069420, T32 AG066574, P30 AG013280, P30 AG066515, U01 DK137097, U19 AG065156