High-Level Production of 2′-Fucosyllactose in Escherichia coli by Systematic Metabolic Engineering
China Agricultural University · Jiangsu Academy of Agricultural Sciences · +1 more institution
Abstract
2′-Fucosyllactose (2′-FL), one of the primary human milk oligosaccharides (HMOs), exerts a pivotal influence on early human development as well as health benefits on other life stages. In this study, a high-yielding strain for 2′-FL production was constructed in Escherichia coli BL21star(DE3) using a systematic metabolic engineering strategy. The α-1,2-fucosyltransferase BKHT from Helicobacter sp. 13S00401-1 was selected as the optimal enzyme for 2′-FL biosynthesis, and the best variant F304W of BKHT was obtained to improve 2′-FL biosynthesis using semirational enzyme modifications. A “push–pull” strategy was implemented to optimize the carbon flux within the 2′-FL biosynthetic pathways. After the enhancement…
Citation impact
- FWCI
- 60.46
- Percentile
- 100%
- References
- 43
Authors
6Topics & keywords
- Metabolic engineering
- Production (economics)
- Enzyme
- Flux (metallurgy)
- Productivity
- Cofactor
- Metabolic pathway
- Strain (injury)