Precision acoustofluidics for high-throughput mechanobiology in suspension cells

Mechanomodulation, the process of altering cellular behavior through applied mechanical forces, plays a critical role in physiological processes and has substantial implications for cancer therapy, immunology, and drug development. However, precise and efficient stimulation of nonadherent cells remains a major challenge, limiting the investigation of mechanotransduction pathways and the development of targeted therapeutics. Here, we developed an acoustofluidic platform named Suspension-cell Targeted Response to Excitation via Acoustofluidic Mechanomodulation (STREAM) to enable precise, high-throughput stimulation of suspension cells. STREAM accomplishes this using 101.14-megahertz high-frequency surface…

• NS
  National Science Foundation

  Award: CMMI-2104295
• NI
  National Institutes of Health

  Award: R01AG084098, R01GM141055, R01GM144417, R01GM145960
• NS
  National Science Foundation Graduate Research Fellowship Program

  Award: 2139754