articleLight Science & ApplicationsJan 4, 2026GOLD OA

Label-free mid-infrared dichroism-sensitive photoacoustic microscopy for histostructural analysis of engineered heart tissues

EPEunwoo ParkDGDong Gyu HwangHCHwanyong ChoiDKD. KimJAJoongho Ahn

Pohang University of Science and Technology · Pohang Iron and Steel (South Korea) · +3 more institutions

PubMed
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Abstract

Many biological tissues, such as cardiac muscle, tendons, and the cornea, exhibit highly organized microstructural alignment that is critical for mechanical and physiological functions. Disruptions in this structural organization are commonly associated with pathological conditions such as fibrosis, infarction, and cancer. However, conventional histological imaging techniques rely on immunofluorescence or histochemical staining, and they evaluate tissue alignment via non-physical 2D gradient-based calculation, which is labor-intensive, antibody-dependent, and prone to variability. Here, we demonstrate label-free mid-infrared dichroism-sensitive photoacoustic microscopy (MIR-DS-PAM), an analytical imaging…

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Topics & keywords

Topics
Keywords
  • Extracellular matrix
  • Photoacoustic imaging in biomedicine
  • Microscopy
  • Tissue engineering
  • Biological tissue
  • Native tissue
  • Confocal microscopy
  • Optical imaging
UN Sustainable Development Goals
  • Decent work and economic growth
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