Fe–N 3 S Single-Atom Nanozyme with Asymmetric Coordination for Ultra-Low-Background Colorimetric Immunoassays
Key Laboratory of Guangdong Province · Fuzhou University · +3 more institutions
Abstract
Although nanocatalysts have advanced highly sensitive immunoassays for low-abundance proteins, the nonspecific catalytic reactions cause high background signals and notable false-positive issues, hindering their further application. Herein, through an asymmetric coordination field modulation strategy, we rationally designed and synthesized an Fe–N3S single-atom nanozyme with a unique peroxidase (POD) catalytic pathway. Specifically, the Fe–N3S nanozyme exhibited high POD-like activity (26.82 U mg–1) and low oxidase (OXD)-like activity (0.018 U mg–1), whereas Fe–N4 nanozymes showed the opposite trend (7.68 U mg–1 for POD-like, 0.19 U mg–1 for OXD-like). Density functional theory (DFT) calculations revealed that…
Citation impact
- FWCI
- 112.52
- Percentile
- 100%
- References
- 40
Authors
8- YZYu Zheng
Key Laboratory of Guangdong Province, Fuzhou University
- MXMingdi XuCorresponding
Key Laboratory of Guangdong Province, Fuzhou University, Fujian University of Technology
- MXMingdi Xu
Key Laboratory of Guangdong Province, Fuzhou University, Fujian University of Technology
- MXMan XuCorresponding
Key Laboratory of Guangdong Province, Fuzhou University, Fujian University of Technology
- MXMan Xu
Key Laboratory of Guangdong Province, Fuzhou University, Fujian University of Technology
Topics & keywords
- Detection limit
- Catalysis
- Nanomaterial-based catalyst
- Chromogenic
- Colorimetry
- Ligand (biochemistry)
- Affordable and clean energy