Nanoinformatics‐Guided Inhibition of blaOXA in Staphylococcus nepalensis using Cysteine‐Conjugated Silver Nanoparticles
University of Central Punjab · University of Ioannina · +6 more institutions
Abstract
Abstract Urinary tract infections, particularly cystitis, are increasingly associated with multidrug‐resistant pathogens, including Escherichia coli , Klebsiella pneumoniae , and Staphylococcus species. This study investigated the molecular characterization of the blaOXA gene encoding oxacillinase type beta‐lactamase in Staphylococcus nepalensis and evaluated its inhibition using nanoinformatics‐guided silver nanoparticles and cysteine‐conjugated silver nanoparticles. The blaOXA gene was amplified by PCR and confirmed by Sanger sequencing. The protein structure was predicted using AlphaFold3 and validated through Ramachandran plot analysis. Silver nanoparticle models were designed using Materials Studio,…
Citation impact
- FWCI
- 37.62
- Percentile
- 99%
- References
- 43
Authors
12Topics & keywords
- Silver nanoparticle
- Molecular dynamics
- Nanoparticle
- Molecular model
- Atomic force microscopy
- Escherichia coli
- Docking (animal)
- Virtual screening