AFG Ameliorates Diabetic Retinopathy through AMPK-Dependent Autolysosomal Pathway: From Network Pharmacology to In Vivo and In Vitro Validation
Jilin Agricultural University · Materials Development (United States) · +4 more institutions
Abstract
Diabetic retinopathy (DR) is a sight-threatening microvascular complication of diabetes. This study investigates the protective effects and mechanisms of arginyl-fructosyl-glucose (AFG) (a hypoglycemic component from red ginseng) against DR. Utilizing network pharmacology and experimental models (HFD/STZ-induced mice and high-glucose-stimulated retinal cells), we demonstrate that AFG alleviates retinal injury by activating AMPK-driven autolysosome biogenesis. This core mechanism inhibits VEGF-mediated vascular hyperpermeability to preserve the blood-retinal barrier while also promoting autophagic flux, reducing lysosomal membrane permeabilization, and ultimately curtailing apoptosis. Our research results…
Citation impact
- FWCI
- 49.03
- Percentile
- 100%
- References
- 54
Authors
9- RHRui-yi Hu
Jilin Agricultural University, Materials Development (United States)
- WSWenya Su
Jilin Agricultural University, Materials Development (United States)
- SQSi-min Qi
Chinese University of Hong Kong
- HSHui Shi
Jilin University, First Hospital of Jilin University
- YZYinan Zheng
Jilin Agricultural University, Materials Development (United States)
Topics & keywords
- Diabetic retinopathy
- Autophagy
- In vivo
- Retinal
- In vitro
- Downregulation and upregulation
- Good health and well-being