Efficient extraction of flavonoids from Cercis chinensis bark using ultrasound-assisted deep eutectic solvents: Process optimization, characterization, kinetic studies, antioxidant and enzyme inhibition activities

This study presents an efficient and eco-friendly approach for extracting flavonoids from Cercis chinensis bark using ultrasound-assisted deep eutectic solvent (UAE-DES) extraction. Among 13 screened DES systems, L -proline-sorbitol (molar ratio 1:2, DES-9) exhibited the maximum extraction yield (51.2 mg/g), significantly outperforming conventional 60 % ethanol extraction. An optimal flavonoid yield of 87.6 mg/g was achieved through the optimization of key parameters (ultrasonic temperature, ultrasonic time and water content) using Response Surface Methodology, with the ideal conditions identified as 42 °C, 40 min and 8 % water. UPLC-Q-Exactive Orbitrap MS analysis revealed that C. chinensis bark flavonoids…

• NN
  National Natural Science Foundation of China

  Award: 21702178
• XU
  Xuchang University

  Award: 2025FB017
• SA
  Science and Technology Department of Henan Province

  Award: 242102310503
• FF
  Foundation for Distinguished Young Talents in Higher Education of Henan
• NC
  National College Students Innovation and Entrepreneurship Training Program

  Award: 202510480021