High yield production of 3-hydroxypropionic acid using Issatchenkia orientalis

Biomanufacturing provides a more sustainable alternative to fossil-based chemical manufacturing. 3-Hydroxypropionic acid (3HP) is a top Department of Energy value-added chemical and precursor to bioplastics, yet cost-effective microbial production remains elusive. Here, we establish the acid-tolerant yeast Issatchenkia orientalis as a robust host for low-pH 3HP biosynthesis. Genome-scale modeling identifies the β-alanine pathway as optimal, offering the highest theoretical yield and lowest oxygen requirement. Thermodynamic analysis confirms its favorability under acidic conditions. Using sequence similarity network analysis, we discover highly active aspartate 1-decarboxylase (PAND), β-alanine-pyruvate…

• UD
  U.S. Department of Energy

  Awards: DE-SC0018420, SC0018420
• UO
  University of Pennsylvania
• PS
  Pennsylvania State University
• OO
  Office of Science

  Award: DE-SC0018420
• IF
  Institute for Computational and Data Sciences, Pennsylvania State University
• BA
  Biological and Environmental Research

  Award: DE-SC0018420