articleFrontiers in Soil ScienceJan 12, 2026GOLD OA

Next-generation sequencing approaches for soil microbiome research

Centre of Advanced Studies · Sechenov University · +1 more institution

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Abstract

DNA sequencing technologies play a key role in modern soil microbiome research, providing a deep understanding of its structure and functional role in ecosystems. 16S rRNA gene, region of 18S-ITS-28S sequencing and shotgun sequencing using modern sequencing technologies (Illumina, Pacific Biosciences (PacBio), Oxford Nanopore Technologies (ONT)) allow us to identify the diversity and dynamics of microbial communities with high accuracy and resolution, which significantly expands our knowledge of biological processes and interactions between microorganisms in the soil. Soil microbiome analysis using sequencing contributes to the development of innovative methods for sustainable agricultural land management,…

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5
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FWCI
72.51
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100%
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260
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Authors

5

Topics & keywords

Keywords
  • Microbiome
  • Metagenomics
  • Shotgun sequencing
  • DNA sequencing
  • Nanopore sequencing
  • Deep sequencing
  • Amplicon sequencing
  • Sustainable agriculture
UN Sustainable Development Goals
  • Zero hunger
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