Integration of imaging-based and sequencing-based spatial omics mapping on the same tissue section via DBiTplus

Spatially mapping the transcriptome and proteome in the same tissue section can profoundly advance our understanding of cellular heterogeneity and function. Here we present Deterministic Barcoding in Tissue sequencing plus (DBiTplus), an integrative multimodal spatial omics approach combining sequencing-based spatial transcriptomics and multiplexed protein imaging on the same section, enabling both single-cell-resolution cell typing and transcriptome-wide interrogation of biological pathways. DBiTplus utilizes spatial barcoding and RNase H-mediated cDNA retrieval, preserving tissue architecture for multiplexed protein imaging. We developed computational pipelines to integrate these modalities, allowing…

• NS
  National Science Foundation

  Awards: 2245575, 2345215
• CF
  Center for Selective C-H Functionalization, National Science Foundation

  Award: 2245575
• NI
  National Institutes of Health

  Awards: RF1MH128876, UH3CA257393, U54AG079759, RM1MH132648, U54AG076043, U01CA294514
• NC
  National Cancer Institute

  Awards: U54CA274509, U54CA268083, R01CA245313