3,4-Dimethoxychalcone-induced caloric restriction inhibits PANoptosis to promote ischemic and diabetic skin flap survival: experimental studies

Ischemic necrosis frequently affects the distal portion of skin flaps, particularly in diabetic patients. PANoptosis, a form of inflammatory programmed cell death, is implicated in vascular damage. This study examined whether 3,4-dimethoxychalcone (3,4-DC), a novel caloric restriction (CR) mimetic, could inhibit PANoptosis and promote the survival of ischemic and diabetic flaps.

Flap viability was evaluated using laser Doppler blood flow imaging and histological analysis. Western blotting and immunofluorescence were used to assess PANoptosis and autophagy. Quantitative PCR was used to measure microRNA levels. Caspase-1 knockout and db/db mice were used to explore the effects of 3,4-DC on pyroptosis and diabetic complications.