Fluorescent dyes in the era of super-resolution imaging: new opportunities, challenges, and evolution
Dalian Institute of Chemical Physics · University of Chinese Academy of Sciences
Abstract
Super-resolution fluorescence imaging (SRI) has redefined the capabilities of optical microscopy, enabling the visualization of biological structures at the nanoscale. This paradigm shift is driven by a unifying principle: achieving spatial resolution by precisely controlling the temporal emission of fluorophores, often referred to as "trading time for space". This strategy relies fundamentally on the precise modulation of fluorescence emission dynamics, which elevates fluorescent dyes from passive markers to active determinants of imaging performance. This shift represents a historic opportunity for fluorescent dyes, one of the oldest classes of synthetic organic molecules, with SRI reinvigorating and…
Citation impact
- FWCI
- 110.16
- Percentile
- 100%
- References
- 92
Authors
4- SWShaowei Wu
Dalian Institute of Chemical Physics, University of Chinese Academy of Sciences
- DHDongjie Hou
Dalian Institute of Chemical Physics, University of Chinese Academy of Sciences
- QQQinglong QiaoCorresponding
Dalian Institute of Chemical Physics
- ZXZhaochao XuCorresponding
Dalian Institute of Chemical Physics, University of Chinese Academy of Sciences
Topics & keywords
- Fluorescence
- Paradigm shift
- Fluorescent protein
- Visualization
- Optical imaging
- Modulation (music)
- Temporal resolution
- Industry, innovation and infrastructure