articleProceedings of the National Academy of SciencesJan 26, 2026HYBRID OA

A temporal and spatial atlas of adaptive immune responses in the lymph node following viral infection

SJShaowen JiangJFJahanara FreedmanMMMadhav MantriVIViviana I. MaymíSAScott A. Leddon

Cornell University · University of California System

PubMed
Indexed incrossrefpubmed

Abstract

The spatial organization of adaptive immune cells within lymph nodes is critical for understanding immune responses during infection and disease. Here, we introduce AIR-SPACE, an integrative approach that combines high-resolution spatial transcriptomics with paired, high-fidelity long-read sequencing of T and B cell receptors. This method enables the simultaneous analysis of cellular transcriptomes and adaptive immune receptor (AIR) repertoires within their native spatial context. We applied AIR-SPACE to mouse popliteal lymph nodes at five distinct time points after Vaccinia virus footpad infection and constructed a comprehensive map of the developing adaptive immune response. Our analysis revealed…

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4
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37.62
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99%
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14

Topics & keywords

Topics
Keywords
  • Germinal center
  • Immune system
  • Acquired immune system
  • Lymph node
  • Lymph
  • Lymphatic system
  • Transcriptome
UN Sustainable Development Goals
  • Good health and well-being
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