Spectroscopic Active Probes for Investigation of Lipid Transformation in Cells and Membranes
Institute of Nuclear Physics, Polish Academy of Sciences
Abstract
Deuterium-labeled lipids provide a powerful means to probe lipid organization, dynamics, and molecular interactions in complex biological systems. In this work, systematic spectroscopic characterization of deuterated fatty acids, sterols, phospholipids, and sphingolipids using Raman and infrared (IR) spectroscopies is presented. Importantly, this study establishes a fundamental spectroscopic reference framework and analytical guidelines for investigating lipid transformation processes in cells and membranes, as all data presented herein are acquired from well-defined lipid standard compounds. The unique C–D stretching region (2300–2000 cm–1), located within the spectroscopically “silent” window and absent in…
Citation impact
- FWCI
- 69.53
- Percentile
- 100%
- References
- 61
Authors
1Topics & keywords
- Raman spectroscopy
- Membrane
- Deuterium
- Infrared
- Sphingolipid
- Infrared spectroscopy
- Biological membrane
- Lipid bilayer