Methods for Tracking Individual Centrioles in Living Cells

The ability to track individual centrioles in living cells represents a pivotal methodology for advancing our understanding of centrosome biology, cell division, and ciliogenesis. This article provides a comprehensive review of the current methodological landscape for centriole tracking, bridging historical approaches with cutting-edge innovations. We begin by outlining the fundamental challenges—including the organelle's sub-diffraction size, dynamic life cycle, and close pairing—that have historically limited observation. The review then details a hierarchical progression of techniques, from foundational static methods like immunofluorescence and electron microscopy to the direct live-cell imaging enabled by…