From sequence to function: Bridging single-molecule kinetics and molecular diversity

Kapanidis, A. N.; Muras, L.; Sreenivasa, Koushik; Hazra, J. P.; Noort, John van; Joo, Chirlmin; Deindl, Sebastian

doi:10.1126/science.adv4503

articleScienceJan 29, 2026Closed access

From sequence to function: Bridging single-molecule kinetics and molecular diversity

ANA. N. Kapanidis LML. Muras KSKoushik Sreenivasa JPJ. P. Hazra JVJohn van Noort

University of Oxford · Uppsala University · +4 more institutions

PubMed

Indexed incrossrefpubmed

Abstract

Biological function is fundamentally determined by nucleic acid and protein sequence. Beyond encoding genetic information, nucleic acids also display complex physicochemical parameters that shape structure, dynamics, and interactions. Understanding how sequence variation sculpts the energetic landscapes underlying these properties requires methods that capture both molecular diversity and dynamic behavior. Single-molecule techniques are ideally suited to this task, but conventional formats remain time and cost intensive. Recent breakthroughs have enabled highly multiplexed approaches for observing molecular dynamics across millions of individual molecules representing thousands of sequences or barcoded…

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Topics

Keywords

Bridging (networking)
Nucleic acid
Sequence (biology)
Function (biology)
Nucleic acid sequence
Molecular dynamics
Directed Molecular Evolution
Base sequence

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