articleAnalytical ChemistryFeb 2, 2026Closed access

Electrochemical Gating of d-Band Engineering in Hierarchically Bridged Dual-Site Nanozymes for Synergistic Cascade Catalysis and Wearable Biosensing

National University of Singapore · Qingdao University of Technology · +4 more institutions

PubMed
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Abstract

Cascade nanozymes for biosensing are fundamentally hampered by diffusion limitations and passive catalytic sites. Herein, we report a strategy of electrochemical gating of d-band engineering within a hierarchically bridged dual-site nanozyme (CuNCs@FeMOP) to achieve dynamic control over cascading catalysis. This architecture spatially confines the ascorbic acid oxidase-mimicking copper nanocluster (CuNC) core and the peroxidase-mimicking iron-based microporous organic polymer (FeMOP) shell, eliminating intermediate diffusion losses. More critically, synergistic electronic coupling via a histidine bridge provides static preoptimization of the Cu and Fe sites’ d-band structure, enhancing their intrinsic…

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