articleBiomassFeb 3, 2026DIAMOND OA

Enzymatic Hydrolysis of Lignocellulosic Biomass: Structural Features, Process Aspects, Kinetics, and Computational Tools

Universidade Federal de Pernambuco · Centro Universitário FEI

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Abstract

This manuscript provides a comprehensive review of the enzymatic hydrolysis of lignocellulosic biomass, emphasizing how chemical composition, structural features, inhibitory compounds, and process configurations collectively influence the conversion of structural polysaccharides into fermentable sugars. Variability among herbaceous, woody, and residual biomasses results in differences in cellulose, hemicellulose, lignin content, and crystallinity, which strongly affect enzyme accessibility. The review discusses key inhibitory mechanisms, including nonproductive cellulase adsorption onto lignin, interference from phenolic derivatives and pretreatment by-products, and inhibition caused by accumulating mono- and…

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