DNA origami vaccines program antigen-focused germinal centers
Massachusetts Institute of Technology · Ragon Institute of MGH, MIT and Harvard · +9 more institutions
Abstract
Priming rare subdominant precursor B cells in germinal centers (GCs) is a central goal of vaccination to generate broadly neutralizing antibodies (bnAbs) against HIV. Multivalent immunogen display on protein nanoparticle scaffolds can promote such responses, but it also generates scaffold-specific B cells that could theoretically limit bnAb precursor expansion in GCs. We rationally designed DNA origami-based virus-like particles (DNA-VLPs) displaying a germline-targeting HIV envelope protein immunogen, which elicited no scaffold-specific antibody responses. Compared with a state-of-the-art clinical protein nanoparticle, these DNA-VLPs increased the expansion of epitope-specific GC B cells relative to…
Citation impact
- FWCI
- 53.59
- Percentile
- 100%
- References
- 98
Authors
25- ARAnna RomanovCorresponding
Massachusetts Institute of Technology
- GAGrant A. Knappe
Massachusetts Institute of Technology
- LRLarance Ronsard
Ragon Institute of MGH, MIT and Harvard
- CAChristopher A. Cottrell
Scripps Research Institute, International AIDS Vaccine Initiative
- YJYiming J. Zhang
Massachusetts Institute of Technology
Topics & keywords
- Immunogen
- Germinal center
- Priming (agriculture)
- DNA origami
- Antibody
- DNA
- Good health and well-being