Molecular insights into the production of extracellular vesicles by plants
Indiana University Bloomington · University of Copenhagen
Abstract
Extracellular vesicles (EVs) produced by Arabidopsis (Arabidopsis thaliana) plants are highly heterogeneous in protein content. To understand the origins of plant EV heterogeneity, we used an unbiased proximity labeling approach to identify proteins and pathways involved in the secretion of distinct EV subpopulations. Proximity labeling, co-immunoprecipitation, and fluorescence microscopy in Nicotiana benthamiana all indicated a general role in EV secretion for EXO70 proteins (a subunit of the exocyst complex) and the immune-related protein RPM1-INTERACTING PROTEIN4 (RIN4). To confirm these hypotheses, we assessed the impact of mutations in various EXO70 family genes and in RIN4 on EV release, as well as…
Citation impact
- FWCI
- 37.62
- Percentile
- 99%
- References
- 137
Authors
14Topics & keywords
- Exocyst
- Endomembrane system
- Arabidopsis
- Mutant
- Secretion
- Arabidopsis thaliana
- Mutation
- Cytokinesis