Copper-carbidopa nanoparticles as highly active laccase-mimics for colorimetric detection and oxidative catalysis of o-phenylenediamine

Abstract Copper-carbidopa nanoparticles (PCD-Cu NPs) have emerged as efficient laccase-mimicking nanozymes with strong potential for biosensing and oxidative catalysis. Their catalytic performance was investigated using o -phenylenediamine (OPD) as a model substrate which undergoes a colorimetric transformation into an oxidized chromophore detectable at 422.0 nm. The reaction followed typical Michaelis-Menten kinetics exhibiting a low apparent K m of 30.0 µM indicating a high binding affinity between the nanozyme and OPD while the V max was determined to be 7.0 µM/min reflecting moderate catalytic turnover. The colorimetric response was linear in the OPD concentration range of 3.0-100.0 µM with a calculated…

• FU
  Future University in Egypt