Single-cell spatial proteomics maps human liver zonation patterns and their vulnerability to disruption in tissue architecture
Max Planck Institute of Biochemistry · National Institutes of Health · +3 more institutions
Abstract
Understanding protein distribution patterns across tissue architecture is crucial for deciphering organ function in health and disease. Here we show the application of single-cell Deep Visual Proteomics to perform spatially resolved proteome analysis of individual cells in native liver tissue. We built a robust framework comprising strategic cell selection and continuous protein gradient mapping, allowing the investigation of larger clinical cohorts. We generated a comprehensive spatial map of the human hepatic proteome by analysing hundreds of isolated hepatocytes from 18 individuals. Among the 2,500 proteins identified per cell, about half exhibited zonated expression patterns. Cross-species comparison with…
Citation impact
- FWCI
- 47.03
- Percentile
- 100%
- References
- 40
Authors
13- CACaroline A. M. WeissCorresponding
Max Planck Institute of Biochemistry
- LALauryn A. Brown
National Institutes of Health, National Cancer Institute, Center for Cancer Research
- LMLucas Miranda
Max Planck Institute of Biochemistry
- PPPaolo Pellizzoni
Max Planck Institute of Biochemistry
- SSSophia Steigerwald
Max Planck Institute of Biochemistry
Topics & keywords
- Proteome
- Proteomics
- Human liver
- Function (biology)
- Proteogenomics
- Vulnerability (computing)
- Liver tissue
- Liver metabolism