Editing strigolactone hormone receptor for robust antiviral silencing in rice
Fujian Agriculture and Forestry University · Nankai University · +10 more institutions
Abstract
The small interfering RNA (siRNA) pathway directs broad-spectrum antiviral defense through RNA silencing so that virulent infection requires efficient suppression of the defense mechanism. Here, we show that strigolactone (SL) hormone signaling promotes antiviral silencing in rice plants by transcriptional activation of RNA-dependent RNA polymerase 1 (RDR1) and RDR6. We demonstrate that protein P3 of the rice grassy stunt virus (RGSV) blocks SL signaling by directly sequestering the receptor DWARF14 from DWARF3. Structural and functional analyses of the P3-DWARF14 complex reveal that the aspartic acid at position 102 (D102) of DWARF14 is essential for the P3 interaction but not for SL perception. Notably, a…
Citation impact
- FWCI
- 60.64
- Percentile
- 100%
- References
- 118
Authors
25Topics & keywords
- RNA silencing
- RNA interference
- Gene silencing
- Small interfering RNA
- RNA-induced silencing complex
- RNA
- Trans-acting siRNA
- Strigolactone
- Zero hunger
Funding
- NNNational Natural Science Foundation of ChinaAwards: 32170167, 32025031
- FAFujian Agriculture and Forestry University
- NSNational Science and Technology Major ProjectAwards: 2024ZD04077, 2023ZD04070
- NKNational Key Research and Development Program of ChinaAwards: 2021YFA1300400, 2023YFF1000500, 2021YFD1400500