articleACS OmegaFeb 26, 2026GOLD OA

Simultaneous Detection and Quantification of Adenine Nucleotides in Mammalian Cultured Cells by HPLC

BKBeatriz KopelFMFernanda M. PradoSLSofia Ligia Guimarães-RamosRDRafael Dias de MouraPDPaolo Di Mascio

Universidade de São Paulo · Instituto de Química y Fisicoquímica Biológicas

PubMed
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Abstract

Adenine nucleotides, including ATP, ADP, ADP-ribose, AMP, NAD+, and NADH, play central roles in cellular homeostasis and are involved in multiple metabolic and signaling pathways. Owing to their broad functional relevance in cell biology, the accurate quantification of these metabolites is essential for diverse research areas such as bioenergetics, cell signaling, and cancer biology. Several analytical methods have been described for the measurement of adenine nucleotides, ranging from enzymatic assays to mass spectrometry-based approaches. In this study, we developed a reverse-phase high-performance liquid chromatography (RP-HPLC) method with UV–vis detection that enables the simultaneous quantification of…

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Keywords
  • High-performance liquid chromatography
  • Nucleotide
  • Enzyme
  • Adenine nucleotide
  • Cell
  • Cell culture
  • Cancer cell
  • Metabolic pathway
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