Experimental study on synergistic pulses improving the tumor microenvironment and assisting immunotherapy

Objective The development and metastasis of tumors depend on the highly complex and heterogeneous tumor microenvironment (TME). Synergistic pulsed electric fields refer to a sequential combination of high-voltage nanosecond pulses and low-voltage microsecond pulses. No systematic research has been reported on the ameliorative effect of this field on the TME and the related mechanisms. This study used tumor-bearing models established with male Balb/c nude mice and male C57BL/6 mice as research subjects. At the molecular, cellular, and tissue levels, this study employed Masson staining, immunofluorescence staining, immunohistochemical staining, and in vivo small animal imaging technology. This study multi-angle…

• NN
  National Natural Science Foundation of China

  Awards: 2023CDJKYJH075, 52477229, 52237010, 2023CDJYGRH-ZD04
• MO
  Ministry of Education of the People's Republic of China
• CS
  Centre Scientifique et Technique du Bâtiment
• RA
  Research and Innovation Foundation
• FR
  Fundamental Research Funds for the Central Universities

  Awards: 2023CDJYGRH, 2023CDJKYJH075, 2023CDJYGRH-ZD04