articleWaterMar 17, 2026GOLD OA

Evaluating the Feasibility of Two Reduced Sulfur Compounds as Energy Sources and Electron Donors for Partial Autotrophic Denitrification: Thiocyanate and Sulfite

Beijing Technology and Business University

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Abstract

Autotrophic denitrification using sulfur compounds is considered an alternative to heterotrophic denitrification for the treatment of organic carbon-deficient wastewaters. However, the stoichiometric characteristics of denitrification using different sulfur species, particularly thiocyanate (SCN−) and sulfite (SO32−), remain poorly understood. Here, partial autotrophic denitrification driven by thiocyanate or sulfite was studied in two batch reactors. The stoichiometry of thiocyanate-oxidizing denitrification was assessed based on valence and ultimate product analysis. No nitrate removal was observed in the sulfite-fed system, indicating that sulfite could not serve as an effective electron donor for…

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