Unstructured transcription factor interactions enable emergent specificity
Howard Hughes Medical Institute · University of California, Berkeley · +2 more institutions
Abstract
How intrinsically disordered regions (IDRs) influence chromatin binding and nuclear organization of transcription factors (TFs) remains unclear. We employed proximity-assisted photoactivation (PAPA), a single-molecule protein-protein interaction sensor, to investigate how IDRs might influence TF interactions with each other and with chromatin in live cells. We found that the Sp1 DNA binding domain (DBD) interacted poorly with chromatin and did not colocalize with Sp1. Weak interaction of the isolated IDR with full-length Sp1 was enhanced by fusion to various unrelated DBDs. Live imaging of Drosophila polytene chromosomes confirmed that an IDR could confer sharp locus specificity on an otherwise nonspecific…
Citation impact
- FWCI
- 47.03
- Percentile
- 100%
- References
- 76
Authors
12- AAAbrar A AbidiCorresponding
Howard Hughes Medical Institute, University of California, Berkeley
- CCClaudia Cattoglio
Howard Hughes Medical Institute, University of California, Berkeley
- NNNatalie N. Tang
University of California, Berkeley
- VBVinson B. Fan
University of California, Berkeley
- GMGina M. Dailey
University of California, Berkeley
Topics & keywords
- Chromatin
- Transcription factor
- DNA
- Polytene chromosome
- Transcription (linguistics)
- Scaffold/matrix attachment region
- Colocalization
- DNA-binding protein