PREreview of "The broad-spectrum RumC1 bacteriocin targets a transient peptidoglycan intermediate of the nascent cell wall"

This Zenodo record is a permanently preserved version of a PREreview. You can view the complete PREreview at https://prereview.org/reviews/19637706. The manuscript by Boyeldieu et al. explores the cell-wall poisoning mechanism of the RumC1 bacteriocin, produced by Ruminococcus gnavus E1. The authors developed an ordered genome-wide mutagenesis strategy in Streptococcus pneumoniae to identify RumC1-resistant mutants. These studies helped confirm RumC1's inhibition of transpeptidase activity, thereby blocking peptidoglycan (PG) synthesis and cell growth. The selective binding of RumC1 to the nascent PG had not been reported before. They also identified the immunity protein RumIc1 from the RumC1 biosynthetic gene…