articleProceedings of the National Academy of SciencesApr 13, 2015BRONZE OA

Mitofusin 2 ablation increases endoplasmic reticulum–mitochondria coupling

University of Padua · National Academies of Sciences, Engineering, and Medicine · +3 more institutions

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Abstract

The organization and mutual interactions between endoplasmic reticulum (ER) and mitochondria modulate key aspects of cell pathophysiology. Several proteins have been suggested to be involved in keeping ER and mitochondria at a correct distance. Among them, in mammalian cells, mitofusin 2 (Mfn2), located on both the outer mitochondrial membrane and the ER surface, has been proposed to be a physical tether between the two organelles, forming homotypic interactions and heterocomplexes with its homolog Mfn1. Recently, this widely accepted model has been challenged using quantitative EM analysis. Using a multiplicity of morphological, biochemical, functional, and genetic approaches, we demonstrate that Mfn2…

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