An ER-Mitochondria Tethering Complex Revealed by a Synthetic Biology Screen
University of California, San Francisco · Howard Hughes Medical Institute · +2 more institutions
Abstract
Communication between organelles is an important feature of all eukaryotic cells. To uncover components involved in mitochondria/endoplasmic reticulum (ER) junctions, we screened for mutants that could be complemented by a synthetic protein designed to artificially tether the two organelles. We identified the Mmm1/Mdm10/Mdm12/Mdm34 complex as a molecular tether between ER and mitochondria. The tethering complex was composed of proteins resident of both ER and mitochondria. With the use of genome-wide mapping of genetic interactions, we showed that the components of the tethering complex were functionally connected to phospholipid biosynthesis and calcium-signaling genes. In mutant cells, phospholipid…
Citation impact
- FWCI
- 30.52
- Percentile
- 100%
- References
- 32
Authors
7- BKBenoı̂t KornmannCorresponding
University of California, San Francisco
- ECErin CurrieCorresponding
University of California, San Francisco
- SRSean R. CollinsCorresponding
Howard Hughes Medical Institute, University of California, San Francisco
- MSMaya Schuldiner
Weizmann Institute of Science
- JNJodi Nunnari
University of California, Davis
Topics & keywords
- Endoplasmic reticulum
- Organelle
- Tethering
- Mitochondrion
- Cell biology
- Membrane contact site
- Biology
- Mutant