A Survey on Temperate Fruit Pests and their Importance in the Highland Areas of North Shewa Zone, Amhara Region, Ethiopia

Fusarium oxysporum f. sp. elaeidis (FOE) a pathogen that causes fusarium wilt disease in oil palm can be detected using polymerase chain reaction (PCR) but very time consuming. Loop-Mediated Isothermal Amplification (LAMP) was used to rapidly detect Fusarium oxysporum f. sp. elaeidis (FOE) in oil palm seedlings. Eight additional Fusarium oxysporum isolates collected from symptomatic oil palm trees (i.e., presumed-FOE as their pathogenicity was not confirmed) and five other non-FOE isolates were sampled from symptomatic mature oil palm trees and tomato respectively to broaden the scope of the research. The identities of FOE, presumed-FOE and non-FOE were established via sequencing. LAMP primers designed for…

• UO
  University of Bath
• UO
  University of Nottingham
• CS
  Commonwealth Scholarship Commission
• AR
  Amhara Regional Agricultural Research Institute