RELION: Implementation of a Bayesian approach to cryo-EM structure determination

RELION, for REgularized LIkelihood OptimizatioN, is an open-source computer program for the refinement of macromolecular structures by single-particle analysis of electron cryo-microscopy (cryo-EM) data. Whereas alternative approaches often rely on user expertise for the tuning of parameters, RELION uses a Bayesian approach to infer parameters of a statistical model from the data. This paper describes developments that reduce the computational costs of the underlying maximum a posteriori (MAP) algorithm, as well as statistical considerations that yield new insights into the accuracy with which the relative orientations of individual particles may be determined. A so-called gold-standard Fourier shell…

• HH
  Howard Hughes Medical Institute
• NI
  National Institutes of Health

  Awards: GM-62580, P41RR02250
• MR
  Medical Research Council

  Award: MC_UP_A025_1013