articleThe Journal of Cell BiologyJul 15, 2002BRONZE OA

Autotaxin has lysophospholipase D activity leading to tumor cell growth and motility by lysophosphatidic acid production

MUMakiko Umezu‐GotoYKYasuhiro KishiATAkitsu TairaKHKotaro HamaNDNaoshi Dohmae

The University of Tokyo · RIKEN · +4 more institutions

PubMed
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Abstract

Autotaxin (ATX) is a tumor cell motility-stimulating factor, originally isolated from melanoma cell supernatants. ATX had been proposed to mediate its effects through 5'-nucleotide pyrophosphatase and phosphodiesterase activities. However, the ATX substrate mediating the increase in cellular motility remains to be identified. Here, we demonstrated that lysophospholipase D (lysoPLD) purified from fetal bovine serum, which catalyzes the production of the bioactive phospholipid mediator, lysophosphatidic acid (LPA), from lysophosphatidylcholine (LPC), is identical to ATX. The Km value of ATX for LPC was 25-fold lower than that for the synthetic nucleoside substrate, p-nitrophenyl-tri-monophosphate. LPA mediates…

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Topics & keywords

Topics
Keywords
  • Autotaxin
  • Lysophosphatidic acid
  • Biology
  • Lysophosphatidylcholine
  • Cell biology
  • Motility
  • Paracrine signalling
  • Autocrine signalling
UN Sustainable Development Goals
  • Good health and well-being
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