Correlation of Daptomycin Bactericidal Activity and Membrane Depolarization in Staphylococcus aureus
Cubist Pharmaceuticals (United States)
Indexed incrossrefpubmed
Abstract
The objective of this study was to further elucidate the role of membrane potential in the mechanism of action of daptomycin, a novel lipopeptide antibiotic. Membrane depolarization was measured by both fluorimetric and flow cytometric assays. Adding daptomycin (5 micro g/ml) to Staphylococcus aureus gradually dissipated membrane potential. In both assays, cell viability was reduced by >99% and membrane potential was reduced by >90% within 30 min of adding daptomycin. Cell viability decreased in parallel with changes in membrane potential, demonstrating a temporal correlation between bactericidal activity and membrane depolarization. Decreases in viability and potential also showed a dose-dependent…
Citation impact
650
total citations
- FWCI
- 12.46
- Percentile
- 100%
- References
- 29
Citations per year
Authors
3Topics & keywords
Topics
Keywords
- Daptomycin
- Staphylococcus aureus
- Microbiology
- Micrococcaceae
- Antibacterial agent
- Chemistry
- Antibiotics
- Bacteria
No related works found for this paper.