Reduction in diversity of the colonic mucosa associated bacterial microflora in patients with active inflammatory bowel disease
University Hospital Schleswig-Holstein · University of Lübeck · +1 more institution
Abstract
BACKGROUND AND AIMS: The intestinal bacterial microflora plays an important role in the aetiology of inflammatory bowel disease (IBD). As most of the colonic bacteria cannot be identified by culture techniques, genomic technology can be used for analysis of the composition of the microflora. PATIENTS AND METHODS: The mucosa associated colonic microflora of 57 patients with active inflammatory bowel disease and 46 controls was investigated using 16S rDNA based single strand conformation polymorphism (SSCP) fingerprint, cloning experiments, and real time polymerase chain reaction (PCR). RESULTS: Full length sequencing of 1019 clones from 16S rDNA libraries (n = 3) revealed an overall bacterial diversity of 83…
Citation impact
- FWCI
- 12.29
- Percentile
- 100%
- References
- 97
Authors
8- SOS. Ott
University Hospital Schleswig-Holstein, University of Lübeck
- MMM Musfeldt
University Hospital Schleswig-Holstein, University of Lübeck
- DFD F Wenderoth
German Center for Infection Research
- JHJ Hampe
University Hospital Schleswig-Holstein, University of Lübeck
- OBO Brant
University Hospital Schleswig-Holstein, University of Lübeck
Topics & keywords
- Inflammatory bowel disease
- Intestinal mucosa
- Medicine
- Inflammatory Bowel Diseases
- Ulcerative colitis
- Disease
- Reduction (mathematics)
- Gastroenterology
- Good health and well-being