Assessing mitochondrial dysfunction in cells

Assessing mitochondrial dysfunction requires definition of the dysfunction to be investigated. Usually, it is the ability of the mitochondria to make ATP appropriately in response to energy demands. Where other functions are of interest, tailored solutions are required. Dysfunction can be assessed in isolated mitochondria, in cells or in vivo, with different balances between precise experimental control and physiological relevance. There are many methods to measure mitochondrial function and dysfunction in these systems. Generally, measurements of fluxes give more information about the ability to make ATP than do measurements of intermediates and potentials. For isolated mitochondria, the best assay is…

• EM
  Ellison Medical Foundation

  Awards: AG-SS-2288-09, SS-2288-09
• WM
  W. M. Keck Foundation
• NI
  National Institutes of Health

  Awards: AG025901, P30 AG025708, P01 AG025901, R01 AG033542, PL1 AG032118