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articleNature MicrobiologyNov 7, 2016HYBRID OA

RETRACTED ARTICLE: Culture of previously uncultured members of the human gut microbiota by culturomics

JLJean‐Christophe LagierSKS. KhelaifiaMTMaryam Tidjani AlouSNS. NdongoNDNiokhor Dione

Centre National de la Recherche Scientifique · Inserm · +9 more institutions

PubMed
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Abstract

Abstract Metagenomics revolutionized the understanding of the relations among the human microbiome, health and diseases, but generated a countless number of sequences that have not been assigned to a known microorganism 1 . The pure culture of prokaryotes, neglected in recent decades, remains essential to elucidating the role of these organisms 2 . We recently introduced microbial culturomics, a culturing approach that uses multiple culture conditions and matrix-assisted laser desorption/ionization–time of flight and 16S rRNA for identification 2 . Here, we have selected the best culture conditions to increase the number of studied samples and have applied new protocols (fresh-sample inoculation; detection of…

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896
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Topics & keywords

Topics
Keywords
  • Metagenomics
  • Biology
  • Microbiome
  • Human Microbiome Project
  • Bacterial taxonomy
  • Human microbiome
  • Archaea
  • Genome
UN Sustainable Development Goals
  • Life in Land
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