SPHIRE-crYOLO is a fast and accurate fully automated particle picker for cryo-EM

Selecting particles from digital micrographs is an essential step in single-particle electron cryomicroscopy (cryo-EM). As manual selection of complete datasets-typically comprising thousands of particles-is a tedious and time-consuming process, numerous automatic particle pickers have been developed. However, non-ideal datasets pose a challenge to particle picking. Here we present the particle picking software crYOLO which is based on the deep-learning object detection system You Only Look Once (YOLO). After training the network with 200-2500 particles per dataset it automatically recognizes particles with high recall and precision while reaching a speed of up to five micrographs per second. Further, we…

• EC
  European Commission

  Awards: FP7/2007-2013, 615984, 2007-2013, FP7/2007
• M
  Max-Planck-Gesellschaft