Precise phylogenetic analysis of microbial isolates and genomes from metagenomes using PhyloPhlAn 3.0
University of Trento · University of California San Diego · +7 more institutions
Abstract
Microbial genomes are available at an ever-increasing pace, as cultivation and sequencing become cheaper and obtaining metagenome-assembled genomes (MAGs) becomes more effective. Phylogenetic placement methods to contextualize hundreds of thousands of genomes must thus be efficiently scalable and sensitive from closely related strains to divergent phyla. We present PhyloPhlAn 3.0, an accurate, rapid, and easy-to-use method for large-scale microbial genome characterization and phylogenetic analysis at multiple levels of resolution. PhyloPhlAn 3.0 can assign genomes from isolate sequencing or MAGs to species-level genome bins built from >230,000 publically available sequences. For individual clades of interest,…
Citation impact
- FWCI
- 41.43
- Percentile
- 100%
- References
- 78
Authors
18Topics & keywords
- Genome
- Metagenomics
- Phylogenetic tree
- Biology
- Clade
- Evolutionary biology
- Phylogenetics
- Phylum
- Life in Land
Funding
- ECEuropean CommissionAwards: 716575, 825410, 818368, H2020, MASTER-818368, H2020-SC1
- MDMinistero dell’Istruzione, dell’Università e della RicercaAwards: H2020, RBFR13EWWI_001
- NINational Institutes of HealthAwards: 1U01CA230551, H2020
- H2Horizon 2020 Framework ProgrammeAwards: 818368, SC1-BHC, H2020-SC1, ONCOBIOME-825410, 825410
- NCNational Cancer InstituteAward: 1U01CA230551