AreTomo: An integrated software package for automated marker-free, motion-corrected cryo-electron tomographic alignment and reconstruction

AreTomo, an abbreviation for Alignment and Reconstruction for Electron Tomography, is a GPU accelerated software package that fully automates motion-corrected marker-free tomographic alignment and reconstruction in a single package. By correcting in-plane rotation, translation, and importantly, the local motion resulting from beam-induced motion from tilt to tilt, AreTomo can produce tomograms with sufficient accuracy to be directly used for subtomogram averaging. Another major application is the on-the-fly reconstruction of tomograms in parallel with tilt series collection to provide users with real-time feedback of sample quality allowing users to make any necessary adjustments of collection parameters.…

• N
  Nvidia
• NC
  Netherlands Centre for Electron Nanoscopy
• UL
  Universiteit Leiden
• NO
  Nederlandse Organisatie voor Wetenschappelijk Onderzoek

  Award: 184.034.014
• LU
  Leids Universitair Medisch Centrum
• NI
  National Institutes of Health

  Awards: 1S10OD021741, R35GM118099, 1S10OD020054
• UO
  University of California, San Francisco