articleeLifeDec 5, 2022GOLD OA

A Bayesian approach to single-particle electron cryo-tomography in RELION-4.0

JZJasenko ZivanovJOJoaquı́n OtónZKZunlong KeAVAndriko von KügelgenEPEuan Pyle

MRC Laboratory of Molecular Biology · University of Basel · +9 more institutions

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Abstract

We present a new approach for macromolecular structure determination from multiple particles in electron cryo-tomography (cryo-ET) data sets. Whereas existing subtomogram averaging approaches are based on 3D data models, we propose to optimise a regularised likelihood target that approximates a function of the 2D experimental images. In addition, analogous to Bayesian polishing and contrast transfer function (CTF) refinement in single-particle analysis, we describe the approaches that exploit the increased signal-to-noise ratio in the averaged structure to optimise tilt-series alignments, beam-induced motions of the particles throughout the tilt-series acquisition, defoci of the individual particles, as well…

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344
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Authors

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Topics & keywords

Topics
Keywords
  • Single particle analysis
  • Electron tomography
  • Software
  • Computer science
  • Algorithm
  • Bayesian probability
  • Cryo-electron microscopy
  • Cryo-electron tomography
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