ROS-mediated lysosomal membrane permeabilization and autophagy inhibition regulate bleomycin-induced cellular senescence

: AT2 cells: type II alveolar epithelial cells; ATG7: autophagy related 7; bEnd.3: mouse brain microvascular endothelial cells; BNIP3L: BCL2/adenovirus E1B interacting protein 3-like; CCL2: C-C motif chemokine ligand 2; CDKN1A: cyclin dependent kinase inhibitor 1A; CDKN2A: cyclin dependent kinase inhibitor 2A; FTH1: ferritin heavy polypeptide 1; γ-H2AX: phosphorylated H2A.X variant histone; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; HUVEC: human umbilical vein endothelial cells; HT22: hippocampal neuronal cell lines; Il: interleukin; LAMP: lysosomal-associated membrane protein; LMP: lysosome membrane permeabilization; MTORC1: mechanistic target of rapamycin kinase complex 1; NAC: N-acetylcysteine; NCOA4:…

• SU
  Shandong University
• NN
  National Natural Science Foundation of China

  Awards: 32270734 to RJ and 81972074 to XY, 81972074
• NS
  Natural Science Foundation of Shandong Province

  Awards: 2023HWYQ, 2022HWYQ-050 to RJ and 2023HWYQ-040 to RL, ZR2019ZD39
• NK
  National Key Research and Development Program of China

  Awards: 2019YFA0112100, 2019YFA0112100 to SF