An image processing pipeline for electron cryo‐tomography in RELION ‐5

Electron tomography of frozen, hydrated samples allows structure determination of macromolecular complexes that are embedded in complex environments. Provided that the target complexes may be localised in noisy, three-dimensional tomographic reconstructions, averaging images of multiple instances of these molecules can lead to structures with sufficient resolution for de novo atomic modelling. Although many research groups have contributed image processing tools for these tasks, a lack of standardisation and interoperability represents a barrier for newcomers to the field. Here, we present an image processing pipeline for electron tomography data in RELION-5, with functionality ranging from the import of…

• PF
  Pittsburgh Foundation
• LT
  Leverhulme Trust
• MR
  Medical Research Council

  Awards: MC_UP_A025_1013, MC_UP_1201/31, MC_UP_1201/6
• BA
  Biotechnology and Biological Sciences Research Council

  Awards: BB/T002670/1, BB/T002670/1
• HE
  H2020 European Research Council

  Award: 852915