Elemental cryo-imaging reveals SOS1-dependent vacuolar sodium accumulation

Increasing soil salinity causes significant crop losses globally; therefore, understanding plant responses to salt (sodium) stress is of high importance. Plants avoid sodium toxicity through subcellular compartmentation by intricate processes involving a high level of elemental interdependence. Current technologies to visualize sodium, in particular, together with other elements, are either indirect or lack in resolution. Here we used the newly developed cryo nanoscale secondary ion mass spectrometry ion microprobe1, which allows high-resolution elemental imaging of cryo-preserved samples and reveals the subcellular distributions of key macronutrients and micronutrients in root meristem cells of Arabidopsis…

• NS
  National Science Foundation
• ÉP
  École Polytechnique Fédérale de Lausanne
• SN
  Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung

  Awards: 205321_212614, 200021_179092
• ET
  Eidgenössische Technische Hochschule Zürich
• UD
  Université de Lausanne
• ER
  European Regional Development Fund
• AE
  Agencia Estatal de Investigación